Study on the gene expression patterns of KANGLAITE in anti-lung metastasis of LA795 mouse.
- Author:
Yu WU
1
;
Yufei YANG
;
Donghua WU
Author Information
- Publication Type:Journal Article
- From: Chinese Journal of Lung Cancer 2003;6(6):473-476
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo screen the differential expression genes of KANLAITE in anti-tumor metastasis.
METHODSmRNA was extracted and purified from the lung of the mouse with LA795 lung metastasis, hybridized respectively on 4 096-gene chip. cDNA microarray was scanned for the fluorescent signals and analyzing difference expression.
RESULTSTwenty-seven differential expressed genes were obtained. Among these genes, 25 were up-regulated and 2 were down-regulated. Twelve of them were Mus musculus cDNA clone. Six genes related with genesis, development and metastasis of tumor.
CONCLUSIONScDNA microarray for analysis of gene expression patterns is a powerful method to identify differential expressed genes. In this study, 6 genes are thought to be associated genes of KANGLAITE in anti-tumor metastasis.
