Establishment of a human large cell lung cancer cell line L9981-nm23-H1.

Author: Liangan CHE ¹ ; Qinghua ZHOU ; Yanping WANG ; Jun CHEN ; Lunxu LIU ; Yang QIN ; Zhilin SUN ; Xiaohe CHEN ; Wen ZHU
Author Information

1. The Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Publication Type:Journal Article
From: Chinese Journal of Lung Cancer 2004;7(3):187-190
CountryChina
Language:Chinese
Abstract:
BACKGROUNDTo establish a human large cell lung cancer cell line L9981-nm23-H1 transfected with wild type nm23-H1 gene.
METHODSpLXSN-nm23-H1-EGFP was constructed by gene clone technique, and L9981-nm23-H1 was established by infected virus of nm23-H1 gene. The DNA and protein expression of nm23-H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot.
RESULTSpLXSN-nm23-H1-EGFP was constructed successfully, and the nm23-H1 cDNA was inducted to L9981 cell. The protein of nm23-H1 could be detected in L9981-nm23-H1 cell.
CONCLUSIONSProtein of nm23-H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.