- Author:
Jie CHEN
1
;
Chunxue BAI
;
Min ZHANG
;
Zhenyi REN
;
Jie HU
Author Information
- Publication Type:Journal Article
- From: Chinese Journal of Lung Cancer 2004;7(3):199-201
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo investigate the expression of aquaporins in human pulmonary adenocarcinoma cell line SPC-A-1.
METHODSThe expressions of aquaporin 1, aquaporin 3, aquaporin 4, and aquaporin 5 in mRNA level and their locations were determined in cell line SPC-A-1 respectively by RT-PCR and immunohistochemistry.
RESULTSThe immunohistochemical stain showed aquaporin 3 and aquaporin 5 located on the membrane of SPC-A-1 cell, but no positive stain of aquaporin 1 and aquaporin 4 was observed. Both aquaporin 3 and aquaporin 5 mRNA expressed in SPC-A-1 cell line, and the expression level of aquaporin 5 mRNA was significantly higher than that of aquaporin 3 mRNA ( P < 0.01). Aquaporin 1 and aquaporin 4 mRNA did not express in SPC-A-1 cell line.
CONCLUSIONSAquaporin 3 and aquaporin 5 express in SPC-A-1 cell, and their roles in water transport of SPC-A-1 cell should be further investigated.