Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria.
- Author:
Wonmok LEE
1
;
Myungsook KIM
;
Dongeun YONG
;
Seok Hoon JEONG
;
Kyungwon LEE
;
Yunsop CHONG
Author Information
- Publication Type:Evaluation Studies ; Original Article ; Research Support, Non-U.S. Gov't
- Keywords: MALDI-TOF MS; VITEK MS; Anaerobic bacteria
- MeSH: Bacteria, Anaerobic/*genetics/isolation & purification; Bacterial Typing Techniques/*instrumentation/*methods; Body Fluids/microbiology; Databases, Genetic; Humans; RNA, Ribosomal, 16S/*analysis/metabolism; Sequence Analysis, DNA; *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From:Annals of Laboratory Medicine 2015;35(1):69-75
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMerieux, France) in the identification of anaerobes. METHODS: We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing. RESULTS: Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level. CONCLUSIONS: The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.
