Application of throat swab nested PCR in the diagnosis of congenital human cytomegalovirus infection in neonates.
- Author:
Xiao-Ting WANG
1
;
Zhi-Bing DONG
;
Li-Ming LUO
;
Min DENG
Author Information
- Publication Type:Journal Article
- MeSH: Cytomegalovirus Infections; congenital; diagnosis; Fetal Blood; virology; Humans; Infant, Newborn; Phosphoproteins; blood; Polymerase Chain Reaction; methods; Viral Envelope Proteins; genetics; Viral Matrix Proteins; blood
- From: Chinese Journal of Contemporary Pediatrics 2013;15(12):1086-1088
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the clinical application value of throat swab nested PCR for detecting active congenital human cytomegalovirus (HCMV) infection in neonates.
METHODSThe throat swabs and umbilical cord blood specimens from 51 neonates were collected for nested PCR assay for HCMV glycoprotein B (gB) gene. Moreover, 18 of them were subjected to a pp65 antigen test.
RESULTSThe sensitivity and specificity of throat swab nested PCR for HCMV gB gene were 67% and 75%, respectively, and the positive and negative predictive values were 57% and 82%, respectively.
CONCLUSIONSThroat swab nested PCR assay for HCMV gB gene is non-invasive, rapid, and highly sensitive for HCMV detection and holds promise as an excellent screening technology for detecting active congenital HCMV infection in neonates.
