Production of β-carotene by metabolically engineered Saccharomyces cerevisiae.
- Author:
Beibei WANG
;
Mingyu SHI
;
Dong WANG
;
Jiaoyang XU
;
Yi LIU
;
Hongjiang YANG
;
Zhubo DAI
;
Xueli ZHANG
- Publication Type:Journal Article
- MeSH:
Basidiomycota;
enzymology;
Farnesyltranstransferase;
genetics;
metabolism;
Hydroxymethylglutaryl CoA Reductases;
genetics;
metabolism;
Metabolic Engineering;
Polyisoprenyl Phosphates;
Saccharomyces cerevisiae;
metabolism;
beta Carotene;
biosynthesis
- From:
Chinese Journal of Biotechnology
2014;30(8):1204-1216
- CountryChina
- Language:Chinese
-
Abstract:
β-carotene has a wide range of application in food, pharmaceutical and cosmetic industries. For microbial production of β-carotene in Saccharomyces cerevisiae, the supply of geranylgeranyl diphosphate (GGPP) was firstly increased in S. cerevisiae BY4742 to obtain strain BY4742-T2 through over-expressing truncated 3-hydroxy-3-methylglutaryl-CoA reductase (tHMGR), which is the major rate-limiting enzyme in the mevalonate (MVA) pathway, and GGPP synthase (GGPS), which is a key enzyme in the diterpenoid synthetic pathway. The β-carotene synthetic genes of Pantoea agglomerans and Xanthophyllomyces dendrorhous were further integrated into strain BY4742-T2 for comparing β-carotene production. Over-expression of tHMGR and GGPS genes led to 26.0-fold increase of β-carotene production. In addition, genes from X. dendrorhous was more efficient than those from P. agglomerans for β-carotene production in S. cerevisiae. Strain BW02 was obtained which produced 1.56 mg/g (dry cell weight) β-carotene, which could be used further for constructing cell factories for β-carotene production.
