Progresses in predicting the crystallizability of proteins.
- Author:
Renbin ZHOU
;
Huimeng LU
;
Dachuan YIN
- Publication Type:Journal Article
- MeSH:
Crystallization;
methods;
Crystallography, X-Ray;
Protein Structure, Tertiary;
Proteins;
chemistry
- From:
Chinese Journal of Biotechnology
2014;30(9):1362-1371
- CountryChina
- Language:Chinese
-
Abstract:
Determination of protein 3-dimensional structure offers very important information in biology researches, especially for understanding protein functions and redundant drug design. The X-ray crystallography is still the main technique for protein structure determination. Obtaining protein crystals is an essential procedure after protein purification in this technique. However, there is only 42% of soluble purified proteins yield crystals by statistics. Experimental verification of protein crystallizability is relatively expensive and time-consuming. Thus it is desired to predict the protein crystallizability by a computational method before starting the experiment. In this paper, combined with our own efforts, some successful in silico methods to predict the protein crystallizability are reviewed.
