Display cellulolytic enzymes on Saccharomyces cerevisiae cell surface by using Flo1p as an anchor protein for cellulosic ethanol production.
- Author:
Chunling MO
;
Yueyue YANG
;
Ning CHEN
;
Xiushan YANG
;
Shen TIAN
- Publication Type:Journal Article
- MeSH:
Aspergillus;
enzymology;
Cellulase;
genetics;
Cellulose;
metabolism;
Cellulose 1,4-beta-Cellobiosidase;
genetics;
Enzymes, Immobilized;
genetics;
Ethanol;
metabolism;
Fermentation;
Glucosidases;
genetics;
Mannose-Binding Lectins;
metabolism;
Protein Binding;
Saccharomyces cerevisiae;
genetics;
metabolism;
Saccharomyces cerevisiae Proteins;
metabolism;
Trichoderma;
enzymology
- From:
Chinese Journal of Biotechnology
2014;30(9):1401-1413
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we constructed a yeast consortium surface-display expression system by using Flo1 as an anchor protein. Endoglucanase II (EGII) and cellobiohydrolase II (CBHII) from Trichoderma reesei, and β3-glucosidase 1 (BGLI) from Aspergillus aculeatus were immobilized on Saccharomyces cerevisiae Y5. We constructed the cellulose-displaying expression yeast consortium (Y5/fEGII:Y5/fCBHII:Y5/fBGLI = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. The displayed cellulolytic enzymes was stabile during the 96-h fermentation. The yeast consortium produced 0.77 g/L ethanol from 10 g/L phosphoric acid swollen cellulose (PASC) within 96 h. The yield (in grams of ethanol produced per gram of carbohydrate consumed) was 0.35 g/g, which correspond to 68.6% of the theoretical yield.
