Optimization of the fermentation conditions for 5-keto-D-gluconic acid production.
- Author:
Boyi LI
;
Haifeng PAN
;
Weirong SUN
;
Yongqing CHENG
;
Zhipeng XIE
;
Jianguo ZHANG
- Publication Type:Journal Article
- MeSH:
Bioreactors;
Fermentation;
Gluconates;
metabolism;
Gluconobacter oxydans;
metabolism;
Glucose;
metabolism;
Industrial Microbiology;
Tartrates;
metabolism
- From:
Chinese Journal of Biotechnology
2014;30(9):1486-1490
- CountryChina
- Language:Chinese
-
Abstract:
Gluconobacter oxydans converts glucose to gluconic acid and subsequently to 5-keto-D-gluconic acid (5-KGA), a precursor of industrially important L(+)-tartaric acid. To increase the yield of 5-KGA, fermentation conditions of 5-KGA production was optimized. Under the optimum medium and culture conditions in the shake flask, the highest 5-KGA production reached 19.7 g/L, increased by 43.8% after optimization. In a 5-L bioreactor, the pH was controlled at 5.5 and dissolved oxygen (DO) at 15%, 5-KGA production reached 46.0 g/L, raised at least 1.3 times than in the shake flask. Glucose feeding fermentation process was further developed, and the highest 5-KGA production of 75.5 g/L with 70% of yield was obtained, 32.0% higher than the highest reported value. Therefore, this newly developed fermentation process provided a practical and effective alternative for the commercial production of 5-KGA, and further of L(+)-tartaric acid.
