Effect of different transfection reagents and injection methods in mice testicular injection on the expression of exogenous gene.
- Author:
Jianjun DAI
;
Xiang LI
;
Caifeng WU
;
Shushan ZHANG
;
Tingyu ZHANG
;
Defu ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Humans;
Indicators and Reagents;
chemistry;
Injections;
methods;
Lipids;
chemistry;
Male;
Mice;
Mice, Transgenic;
Plasmids;
Polymerase Chain Reaction;
Seminiferous Tubules;
Spermatozoa;
Testis;
Transfection
- From:
Chinese Journal of Biotechnology
2014;30(10):1522-1530
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to study the effect of three different transfection reagents (Lipofectamine™ LTX & PLUS™, Lipofectamine 2000 and Nano-PAMAM-D) and three different testicular injection methods (rete testicular injection, seminiferous tubules injection and testicular interstitial injection) on the efficiency of production transgenic mice. After the mixtures of plasmid DNA (pEFP-C1) and transfection reagent were injected with different testicular injection methods, the sperm density, vitality, positive sperm rates and PCR positive transgenic mice rate were examined 30 days after injection. The results showed that the damage degree from slight to serious of three transfection reagents was Lipofectamine™ LTX & PLUS™, Lipofectamine 2000, and PAMAM-D. The sperm positive rates with green fluorescence of these three groups were 35.65%±0.69%, 12.86%±0.35% and 10.04%±0.20%, respectively. The PCR positive rates of transgenic newborn mice were 29.17%, 13.70% and 5.88%, respectively. Among the groups of different testicular injection methods, the damage degree from slight to serious was rete testicular injection, seminiferous tubules injection, and testicular interstitial injection, whereas the sperm positive rates with green fluorescence were 35.13%, 15.13%, and 0%, respectively. The PCR positive rates of transgenic newborn mice among different testicular injection groups were 33.3%, 12.5%, and 0.0%. The combination of rete testicular injection and Lipofectamine™ LTX & PLUS™ had the lowest toxicity and highest transgenic efficiency in the production of transgenic mice.
