BAMBI inhibits porcine preadipocyte differentiation by facilitating ERK1/2 phosphorylation.
- Author:
Yin MAI
;
Zhenyu ZHANG
;
Peiyue DONG
;
Hao YANG
;
Gongshe YANG
;
Shiduo SUN
- Publication Type:Journal Article
- MeSH:
Adipocytes;
cytology;
Adipogenesis;
Animals;
Cell Differentiation;
Membrane Proteins;
metabolism;
Mitogen-Activated Protein Kinase 1;
metabolism;
Mitogen-Activated Protein Kinase 3;
metabolism;
PPAR gamma;
metabolism;
Phosphorylation;
Swine
- From:
Chinese Journal of Biotechnology
2014;30(10):1531-1540
- CountryChina
- Language:Chinese
-
Abstract:
To study the role of BAMBI in adipogenesis, we constructed lentivirus interfering vector targeting on porcine BAMBI, packaged and infected the porcine preadipocyte. The differentiation state of preadipocyte was detected by Oil Red O staining and Oil Red O extraction assay and the expression levels of adipogenic marker genes were detected by Real-time qPCR and Werstern bloting. Results show that BAMBI expression was significant decreased after lentivirus infection, which was repressed more than 60% by shRNA2. Moreover, knockdown BAMBI increased the lipid accumulation of porcine preadipocyte and improved the expression of PPARγ (peroxisome proliferator-activated receptorγ) and ap2 (adipocyte protein 2). In summary, these data indicated that BAMBI inhibited adipocyte differentiation by facilitating the phosphorylation of ERK1/2.
