Expression of limulus Factor C in silkworm larvae by Bac-to-Bac/BmNPV baculovirus expression system.
- Author:
Jing QI
;
Tao LIU
;
Zhen LI
;
Chengliang GONG
;
Haiping WU
;
Chun ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Arthropod Proteins;
biosynthesis;
Baculoviridae;
Bombyx;
metabolism;
Enzyme Precursors;
biosynthesis;
Genetic Vectors;
Larva;
metabolism;
Recombinant Proteins;
biosynthesis;
Serine Endopeptidases;
biosynthesis
- From:
Chinese Journal of Biotechnology
2014;30(10):1594-1601
- CountryChina
- Language:Chinese
-
Abstract:
Limulus Factor C, a serine protease zymogen from the amoebocytes of the limulus, has high affinity for endotoxin. When Factor C is activated by endotoxin, it hydrolyses artificial tripeptide substrate and measurable products are released, so it can be used as an alternative reagent for endotoxin analysis. Factor C gene of Tachypleus tridentatus was obtained through RT-PCR and the recombinant protein was expressed by Bac-to-Bac/BmNPV baculovirus expression system in silkworm larvae. The activity of Factor C was detected with diluted serum of silkworm larvae, and the sensitivity of endotoxin detected was 0.2 EU/mL when the serum was diluted at 1:500. The silkworm larvae expressed limulus Factor C could be used to develop a new low-cost endotoxin test reagent.
