Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens.
- Author:
Hongli LIN
;
Shenda HOU
;
Song WANG
;
Yupeng WANG
;
Yunyan LUANI
;
Xilin HOU
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
blood;
Antibody Formation;
Birnaviridae Infections;
prevention & control;
veterinary;
Chickens;
Infectious bursal disease virus;
Lactobacillus casei;
Poultry Diseases;
prevention & control;
Recombinant Proteins;
immunology;
Viral Structural Proteins;
immunology;
Viral Vaccines;
immunology
- From:
Chinese Journal of Biotechnology
2014;30(11):1679-1690
- CountryChina
- Language:Chinese
-
Abstract:
In order to determine immunogenicity and protective effect in chickens, we used the IBDV (Infectious bursal disease virus)-Vp2/Lactobacillus casei as antigen transfer system. First, the immunized and control chickens were challenged by IBDV/DQ at lethal dose to determine the protective ratio. Second, chickens were orallyand intranasally vaccinated twice with 10(9) CFU/mL pLA-VP2/L. casei, pLA/L. casei and PBS as negativecontrol and commercial vaccine as positive control. The bursa injury and the lesion score wererecorded post challenge. The level of specific IgG and sIgA in pLA-VP2/L. casei and positive control groups was significantly higher than that in negativecontrol groups. The protection efficacy in pLA-VP2/L. casei oral group was higher than that inintranasal group. The SI. of pLA-VP2/L. casei oral group was significant higher than other groups. The lesion score indicated the pLA-VP2/L. casei was safer than commercial vaccine for bursa. Collectively, the pLA-VP2/L. casei could be a vaccine candidate for IBDV.
