Purification of enramycin by macroporous resin adsorption and reversed phase chromatography purification.
- Author:
Wu JIAXIN
;
Huang YONGDONG
;
Qi PENG
;
He JIHONG
;
Li PING
;
Zhang GUODONG
;
Zhao MEIXIAN
- Publication Type:Journal Article
- MeSH:
Adsorption;
Anti-Bacterial Agents;
isolation & purification;
Chromatography, Reverse-Phase;
methods;
Peptides;
isolation & purification
- From:
Chinese Journal of Biotechnology
2014;30(11):1701-1708
- CountryChina
- Language:Chinese
-
Abstract:
Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50: 50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70: 30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with a high purity.
