Microbial community in the Anammox process of thermal denitration tail liquid.
- Author:
Jin LI
;
Deshuang YU
;
Dan ZHAO
;
Xiaochen WANG
- Publication Type:Journal Article
- MeSH:
Ammonia;
chemistry;
Bacteria;
Phylogeny;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S;
Sewage;
microbiology
- From:
Chinese Journal of Biotechnology
2014;30(12):1865-1875
- CountryChina
- Language:Chinese
-
Abstract:
An anaerobic sequencing batch reactor (ASBR) was used to treat thermal denitration tail liquid and microbial community was studied. Activated sludge was taken from the reactor for scanning electron microscope analysis. The images showed that the dominant cells in the flora were oval cocci. Its diameter was about 0.7 μm. Through a series of molecular biology methods such as extracting total DNA from the sludge, PCR amplification, positive clone authentication and sequencing, we obtained the 16S rDNA sequences of the flora. Phylogenetic tree and clone library were established. The universal bacteria primers of 27F-1492R PCR amplification system obtained 85 clones and could be divided into 21 OTUS. The proportions were as follows: Proteobacteria 61.18%; Acidobacteria 17.65%; Chlorobi 8.24%; Chlorofexi 5.88%; Gemmatimonadetes 3.53%; Nitrospirae 2.35% and Planctomycetes 1.18%. The specific anammox bacterial primers of pla46rc-630r and AMX368-AMX820 PCR amplification system obtained 45 clones. They were divided into 3 OTUS. Candidatus brocadia sp. occupied 95.6% and unknown strains occupied 4.4%.
