Construction and characterization of an epitope-mutated Asia 1 type foot-and-mouth disease virus.
- Author:
Yan ZHANG
;
Yonghao HU
;
Fan YANG
;
Bo YANG
;
Songhao WANG
;
Zixiang ZHU
;
Haixue ZHENG
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
DNA, Complementary;
Epitopes;
genetics;
Foot-and-Mouth Disease Virus;
genetics;
Immune Sera;
Neutralization Tests;
Transfection
- From:
Chinese Journal of Biotechnology
2015;31(1):96-104
- CountryChina
- Language:Chinese
-
Abstract:
To generate an epitope-mutated foot-and-mouth disease virus (FMDV) as a marker vaccine, the infectious clone pAsia 1-FMDV containing the complete genomic cDNA of Asia 1 type FMDV was used as backbone, the residues at positions 27 and 31 in the 3D gene were mutated (H27Y and N31R). The resulting plasmid pAsia 1-FMDV-3DM encoding a mutated epitope was transfected into BHK-21 cells and the recombinant virus rAsia 1-3DM was rescued. The recombinant virus showed similar biological characteristics comparable with the parental virus. In serological neutralization test the antisera against recombine virus have a good reactivity with parental virus. The antisera against the mutant virus were shown to be reactive with the mutated epitope but not the wild-type one. The results indicated that the two virus strains could be distinguished by western blotting using synthetic peptides. This epitope-mutated FMDV strain will be evaluated as a potential marker vaccine against FMDV infections.
