Inhibition of replication and transcription of WSN influenza A virus by IFIT family genes.
- Author:
Lidan HOU
;
Jing LI
;
Hongren QU
;
Limin YANG
;
Yajun CHEN
;
Qianqian DU
;
Wenjun LIU
- Publication Type:Journal Article
- MeSH:
HEK293 Cells;
Humans;
Influenza A virus;
physiology;
Influenza, Human;
genetics;
Intracellular Signaling Peptides and Proteins;
genetics;
Proteins;
genetics;
Virus Replication
- From:
Chinese Journal of Biotechnology
2015;31(1):123-134
- CountryChina
- Language:Chinese
-
Abstract:
IFIT family genes are a kind of interferon stimulated genes (ISGs), and play important roles in antiviral sector and immunity regulation. To study the regulatory effect of IFIT family genes during influenza A virus (IAV) infection, we used RNA-sequencing analysis (RNA-Seq) technique and found that when 293T cells were infected by A/WSN/33 (WSN), the concentration of IFIT family genes were increased. Further study reveals that overexpression of IFIT2 or IFIT3 could inhibit IAV replication and transcription, and cause the dose-dependent inhibition of polymerase activity of vRNP. In addition, IFIT2 and IFIT3 encoding protein could colocalize with NS1 in 293T cells infected by WSN, indicating that they might interact with each other. The results suggest that IFIT family genes can inhibit the replication and transcription of IAV, which contributes to our understanding of the regulatory effect of host factors during influenza virus infection.
