Analysis of HPA1-16 and HLA-A, B gene polymorphisms among ethnic Han population from Shandong.
- Author:
Yi ZHANG
1
;
Yuan YU
;
Wenben QIAO
;
Yan LIU
;
Juan ZHOU
;
Jianhong XU
;
Bing FAN
;
Liyue JIANG
;
Wenhua LIANG
;
Chuanfu ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Antigens, Human Platelet; genetics; Asian Continental Ancestry Group; genetics; statistics & numerical data; Blood Donors; China; Female; Gene Frequency; Genetics, Population; Genotype; HLA-A Antigens; genetics; HLA-B Antigens; genetics; Humans; Linkage Disequilibrium; Male; Polymorphism, Genetic
- From: Chinese Journal of Medical Genetics 2016;33(5):690-693
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the polymorphisms of human platelet antigen (HPA) 1-16 and human leukocyte antigen (HLA)-A and -B loci among ethnic Han population from Shandong.
METHODSA total of 588 samples from platelet donors were genotyped for the above loci with sequence-specific primer PCR and sequence-specific oligonucleotide probe PCR.
RESULTSThe frequencies of HPA-la, -1b, HPA-2a, -2b, HPA-3a, -3b, HPA-4a, -4b, HPA-5a, -5b, HPA-6a, -6b, HPA-15a, -15b were 0.9974, 0.0026, 0.9456, 0.0544, 0.5417, 0.4583, 0.9983, 0.0017, 0.9889, 0.0111, 0.9903, 0.0097, 0.5434 and 0.4583, respectively. The HPA-7-14 and HPA-16 showed no heterozygosity as the b allele was not detected in such loci. The most common genotypic combination for HPA was HPA-(1,4,7-14,16,17) aa-2aa-3ab-5aa -6aa-15ab (0.1820). HLA-A2 (0.3070) and HLA-B13 (0.1361) demonstrated the highest frequencies at their respective loci.
CONCLUSIONThe HPA and HLA loci are highly polymorphic among ethnic Hans from Shandong. The distribution of HPA polymorphisms also shows a great ethnic and territorial difference. It is important to construct regional database for the genotypes of HPA and HLA loci for platelet donors.
