Combined G-banded karyotyping and multiplex ligation-dependent probe amplification for the detection of chromosomal abnormalities in fetuses with congenital heart defects.
- Author:
Yang LIU
1
;
Jiansheng XIE
;
Qian GENG
;
Zhiyong XU
;
Weiqin WU
;
Fuwei LUO
;
Suli LI
;
Qin WANG
;
Wubin CHEN
;
Hongxi TAN
;
Hu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Aberrations; Chromosome Banding; Chromosome Disorders; diagnosis; genetics; DNA Copy Number Variations; Female; Fetal Diseases; diagnosis; genetics; Genetic Testing; methods; Heart Defects, Congenital; diagnosis; genetics; Humans; Karyotyping; methods; Multiplex Polymerase Chain Reaction; methods; Pregnancy; Prenatal Diagnosis; methods; Reproducibility of Results; Sensitivity and Specificity
- From: Chinese Journal of Medical Genetics 2017;34(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the value of G-banded karyotyping in combination with multiplex ligation-dependent probe amplification (MLPA) as a tool for the detection of chromosomal abnormalities in fetuses with congenital heart defects.
METHODSThe combined method was used to analyze 104 fetuses with heart malformations identified by ultrasonography. Abnormal findings were confirmed with chromosomal microarray analysis (CMA).
RESULTSNineteen (18%) fetuses were found to harbor chromosomal aberrations by G-banded karyotyping and MLPA. For 93 cases, CMA has detected abnormalities in 14 cases including 10 pathogenic copy number variations (CNVs) and 4 CNVs of uncertain significance (VOUS). MLPA was able to detect all of the pathogenic CNVs and 1 VOUS CNV.
CONCLUSIONCombined use of G-banded karyotyping and MLPA is a rapid, low-cost and effective method to detect chromosomal abnormalities in fetuses with various heart malformations.
