Serological and molecular study of three cases with a rare Bx02 blood group.
- Author:
Bin HAN
1
;
Peiyan LIU
;
Xiaohua LIU
;
Zhihui FENG
Author Information
- Publication Type:Case Reports
- MeSH: ABO Blood-Group System; genetics; Adult; Alleles; Base Sequence; Blood Grouping and Crossmatching; methods; Exons; genetics; Female; Genotype; Humans; Male; Mutation; Phenotype; Polymerase Chain Reaction; methods; Sequence Analysis, DNA; methods; Young Adult
- From: Chinese Journal of Medical Genetics 2017;34(1):65-67
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the genotypes of three blood samples suspected as B subtype through DNA sequencing.
METHODSThe samples were first genotyped with PCR-SSP. Exons 6 and 7 of the ABO genes were subjected to PCR, direct sequencing, and cloned sequencing to determine the genotypes.
RESULTSSerological results of the three samples were similar, with red cells being weakly agglutinatable by anti-B and serum containing anti-B. The samples were preliminarily genotyped as B/O1. Sequencing analysis showed that all three samples contained an O allele and a 905A>G mutation of the B gene, which was previously defined as Bx02.
CONCLUSIONThrough sequencing analysis, the three samples typed as B subtype with serological testing were identified as Bx phenotype. The genotype of samples 1 and 2 was Bx02/O101, and that of sample 3 was Bx02/O102.
