Relations of synovial angiogenesis and PTEN/PI3K/AKT signaling pathway in rats with adjuvant arthritis.
- Author:
Xiao-jun ZHANG
;
Jian LIU
;
Lei WAN
;
Yue SUN
;
Fang WANG
;
Ya-jun QI
;
Chuan-bing HUANG
- Publication Type:Journal Article
- MeSH: Animals; Arthritis, Experimental; physiopathology; Hypoxia-Inducible Factor 1, alpha Subunit; physiology; Neovascularization, Pathologic; etiology; PTEN Phosphohydrolase; physiology; Phosphatidylinositol 3-Kinases; physiology; Proto-Oncogene Proteins c-akt; physiology; Rats; Rats, Sprague-Dawley; Signal Transduction; physiology
- From: China Journal of Orthopaedics and Traumatology 2015;28(1):71-74
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the change of PTEN/PI3K/AKT pathway hypoxia-inducible factor (HIF-1α), vascular endothelial growth factor (VEGF) in rats with adjuvant arthritis and to explore the mechanism of neovasculization in rheumatoid arthritis.
METHODSThirty rats were randomly divided into normal control group and model control group. The model control group were established the model of adjuvant arthritis using Freund's complete adjuvant. At 19 days after modeling, the expression of microvascular density (MVD), HIF-1α, VEGF were detected by ELISA assay and PTEN, PI3K, AKT were detected by Werstern Blotting.
RESULTSCompared with the normal control group, paw swelling, arthritic index were increased, and the expression of MVD, VEGF, HIF-1α of serum, PI3K, AKT of synovial tissue were significantly increased, PTEN was significantly decreased in model control group. PI3K, HIF-1α were positively correlated with MVD; VEGF, AKT were positively correlated with paw swelling; PTEN was negatively correlated with the arthritis index; HIF-1α was positively correlated with VEGF; PI3K was positively correlated with AKT, PTEN was negatively correlated with PI3K, AKT, VEGF.
CONCLUSIONImbalance of PTEN/PI3K/AKT pathway in rats with adjuvant arthritis is one of the mechanisms of synovial neovasculization.