Effects of Chansu injection on transplanting-tumor models S180 in mice and human colon cancer HT-29 in nude mice.
- Author:
Zhen-huai YANG
1
;
Yong-ju LIANG
;
Jie-wen GUO
;
Jing-qiang PAN
;
Yan DING
;
Guan-ning LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bufanolides; pharmacology; Drugs, Chinese Herbal; therapeutic use; Female; HT29 Cells; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Inbred Strains; Mice, Nude; Sarcoma 180; drug therapy; Xenograft Model Antitumor Assays
- From: Chinese Journal of Gastrointestinal Surgery 2005;8(2):169-171
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the antitumor effects of Chansu injection on transplanting- tumor of S(180 ) in mice and human colon cancer HT-29 in nude mice.
METHODSUsing transplanting- tumor models of S(180 ) in mice and human colon cancer HT-29 in nude mice,the tumor inhibitive ratio(IR) of Chansu injection was determined and apoptosis was microscopically observed.
RESULTSCompared with tumor-negative control groups, IR at different dosage of Chansu in models of S(180) and HT-29 was 19.1% - 38.2% and 9.5% - 15.8% respectively,there was a dose-dependent relationship in models of S ( 180) (P< 0.05) and HT- 29 (P> 0.05). The tumor growth was markedly inhibited by cyclophosphamide (CTX) in model of S( 180) with IR of 70.7% and in model of HT-29 with IR of 67.1%, compared with control groups, both P< 0.01; apoptosis induced by CTX was markedly observed by in microscope examination. No significant side effects were shown in the study group.
CONCLUSIONSChansu injection can significantly inhibit tumor growth in model of S(180), but not in model of HT- 29. Different type of tumor has different drug-sensitivity.
