Prediction of lymph node metastasis with binary logistic regression in gastric carcinoma.
- Author:
Jun-qiang CHEN
1
;
Wen-hua ZHAN
;
Yu-long HE
;
Shi-rong CAI
;
Jun-sheng PENG
;
Zheng-xuan CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; Humans; Logistic Models; Lymph Nodes; pathology; Lymphatic Metastasis; pathology; NM23 Nucleoside Diphosphate Kinases; metabolism; Neoplasm Staging; Prognosis; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms; metabolism; pathology; Syndecan-1; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2005;8(5):436-439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate more specific markers to predict the lymph node metastasis in gastric carcinoma.
METHODSThe expression of heparanase mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in 43 cases with gastric cancer. The expressions of CD44V6, MMP-7, nm23 and syndecan-1 protein were examined by streptavidin-peroxidase (SP) two-step method. Clinicopathological features influencing lymphatic metastasis such as age,sex,tumor size,tumor location, Borrmann classification, histological type, differentiation and serosal infiltration were also analyzed.
RESULTSTwenty-seven cases (62.8%) in 43 gastric cancer patients had lymphatic metastasis. The incidence of metastatic lymph nodes was(36.3 +/- 30.8)%. The median incidence was 19%. Univariate analysis showed that tumor size, serosal infiltration, expressions of heparanase mRNA, CD44V6, nm23 and syndecan-1 protein were risk factors for lymph node metastasis in gastric carcinoma. Multivariate analysis showed expressions of nm23 and syndecan-1 protein, serosal infiltration were independent factors for lymph node metastasis.
CONCLUSIONGastric cancer with serosal infiltration, positive expressions of nm23 and syndecan-1 has greater possibility of lymph node metastasis.
