Antisense RNA targeting survivin enhances the chemosensitivity of LOVO/Adr cells to taxotere.

Yan-jun Lian; Dao-da Chen; Tao Huang

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Antisense RNA targeting survivin enhances the chemosensitivity of LOVO/Adr cells to taxotere.

Author: Yan-jun LIAN ¹ ; Dao-da CHEN ; Tao HUANG
Author Information

1. Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. lianalian@163.com
Publication Type:Journal Article
MeSH: Apoptosis; drug effects; Cell Line, Tumor; Colonic Neoplasms; drug therapy; Drug Resistance, Neoplasm; Drug Screening Assays, Antitumor; Humans; Microtubule-Associated Proteins; genetics; pharmacology; RNA, Antisense; genetics; RNA, Messenger; genetics; Taxoids; pharmacology; therapeutic use; Transfection
From: Chinese Journal of Gastrointestinal Surgery 2005;8(5):455-458
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the ability of antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin targeting survivin gene to inhibit survivin expression and enhance the sensitivity to taxotere in multidrug resistant colon carcinoma cell line LOVO/Adr.
METHODSThe antisense RNA eukaryotic plasmid pcDNA3.0/survivin was transfected into LOVO/Adr cells by lipofectamine. The expression of survivin mRNA was measured using RT-PCR. After treated with taxotere, MTT assay and flow cytometry were used to evaluate the proliferation inhibition and apoptosis of LOVO/Adr cells.
RESULTSThe expression of survivin mRNA in LOVO/Adr cells transfected with pcDNA3.0/survivin was down-regulated in a time- dependent manner. The inhibitory rate of taxotere (0.5 micromol/L) was (37.3 +/- 2.9)% in pcDNA3.0/survivin transfected cells, significantly higher than (21.9 +/- 2.3)% and (21.1 +/- 1.9)% in pcDNA3.0 transfected and untransfected control cells respectively (P< 0.01). The apoptosis rate of taxotere was (28.7 +/- 1.7)% in pcDNA3.0/survivin transfected cells,significantly higher than (13.4 +/- 1.6)% and (14.3 +/- 1.8)% in pcDNA3.0 transfected and untransfected cells respectively.
CONCLUSIONThe antisense RNA eukaryotic expression plasmid pcDNA3.0/survivin could down-regulate the expression of survivin gene and enhance the chemosensitivity of LOVO/Adr cells to taxotere, which may provide a novel therapy for colon carcinoma.