Expression of glutathione S-transferase-pi and DNA topoisomerase II alpha and their implications in colorectal carcinoma.
- Author:
Xi-long OU
1
;
Wei-hao SUN
;
Ming XU
;
Da-zhong CAO
;
Qian YU
;
Ting YU
;
Yun-liang SUN
;
Jie JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Antigens, Neoplasm; metabolism; Colorectal Neoplasms; metabolism; pathology; DNA Topoisomerases, Type II; metabolism; DNA-Binding Proteins; metabolism; Female; Glutathione S-Transferase pi; metabolism; Humans; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Staging
- From: Chinese Journal of Gastrointestinal Surgery 2005;8(6):524-527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of GST-pi and Topo II-alpha, and their relationships with clinicopathological parameters in colorectal carcinoma.
METHODSThe expression of GST-pi and Topo II-alpha were detected by avidin-biotin-peroxide complex (ABC) method in tumor specimens, matched paratumor tissues from 60 cases with colorectal carcinoma and normal colonic tissues from 15 cases.
RESULTSThe expression rates of GST-pi and Topo II-alpha were 90.0% and 86.7% respectively in tumor tissues, significantly higher than those in matched paratumor tissues and normal tissues (P< 0.01). The expressions of GST-pi and Topo II-alpha were associated with cellular differentiation, Dukes stage and lymph node metastasis (all P< 0.01), but not with tumor size and histological type (all P > 0.05). The expression level of GST-pi was significantly higher in poorly differentiated tumors than that in well differentiated tumors. The expression level of Topo II-alpha in well-differentiated tumors were stronger than that in poorly differentiated tumors.
CONCLUSIONSThe detection of GST-pi and Topo II-alpha expressions may be helpful to judge the malignant behavior, metastasis and prognosis in human colorectal carcinoma.
