Endothelial nitric oxide synthase gene transfected endothelial cells inhibit smooth muscle cell proliferation and platelet aggregation early in vitro.
- Author:
Guan-Hua XUE
1
;
Ji-Wei ZHANG
;
Lan ZHANG
;
Hao ZHANG
;
Bai-Gen ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Communication; Cell Proliferation; Coculture Techniques; Dogs; Endothelial Cells; cytology; Endothelium, Vascular; cytology; Myocytes, Smooth Muscle; cytology; Nitric Oxide; metabolism; physiology; Nitric Oxide Synthase Type III; genetics; Platelet Aggregation; physiology; Transfection
- From: Chinese Journal of Surgery 2004;42(22):1349-1352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo test the function of endothelial cells transfected eNOS gene.
METHODSTo test the function of eNOS-overexpressing endothelial cells inhibiting smooth muscle cell proliferation and platelet aggregation in vitro by colorimetry, MTT and (3)H-TdR permeate, respectively, in four different endothelial cells groups.
RESULTSThe platelet aggregation is significantly decreasing between the groups of EC and eNOS at the different time (P < 0.05), is 42.2 and 32.6 separately at the time of 120 h. The SMC proliferation is significantly decreasing between the groups of EC and eNOS at the different time (P < 0.05). It is 0.28 and 0.22 by MTT test, 4691 and 3995 by (3)H-TdR permeate separately at the time of 120 h.
CONCLUSIONSeNOS-overexpressing endothelial cells inhibited significantly smooth muscle cells proliferation and platelet aggregation in vitro; which shows powerful effect 48 hours post transfection and lasts up to 120 hours at the least; and were held back by L-NAME.
