The expression and significance of hypoxia-inducible factor-1 alpha and related genes in abdominal aorta aneurysm.
- Author:
Xin-hua HU
1
;
Jun YANG
;
Cheng-wei LIU
;
Zhi-shen ZHANG
;
Qiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Aortic Aneurysm, Abdominal; etiology; genetics; metabolism; Caspase 3; Caspases; biosynthesis; Female; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Immunohistochemistry; Male; Middle Aged; Neovascularization, Pathologic; pathology; RNA, Messenger; genetics; Transcription Factors; biosynthesis; genetics; Vascular Endothelial Growth Factor A; biosynthesis
- From: Chinese Journal of Surgery 2004;42(24):1509-1512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of hypoxia-inducible factor (HIF)-1alpha and related genes in abdominal aorta aneurysm (AAA) and explore the underlying pathogenesis.
METHODSTwenty-two AAA specimens were collected and 5 normal abdominal aorta tissue were used as control. Northern blot, western blot and immunohistochemistry were used to evaluated the expression of HIF-1alpha mRNA and protein product. Western blot and immunohistochemistry method were also used to determine the expression of vascular endothelial growth factor (VEGF) and caspase-3. Microvessel density (MVD) was studied by immunohistochemistry stain of CD34.
RESULTSThe expression of HIF-1alpha mRNA and protein product were significantly higher in AAA than that in normal abdominal aorta (P < 0.01). The expression of VEGF and caspase-3 were also higher in AAA and both had a significantly positive relationship with HIF-1alpha expression (P < 0.01). Most of the positive cells located in VSMC and adventitia of AAA. The MVD counts were higher in AAA.
CONCLUSIONHIF-1alpha may have an important role the development of AAA, which maybe obtained by regulating the expression of VEGF or caspase-3.
