Influence of serum containing Liuwei Dihuang decoction (see text) on proliferation of osteoblasts under stretch-stress environment.
- Author:
Wan CHENG
1
;
Xiao-Kang TANG
;
Hang YING
;
Min LI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Male; Osteoblasts; drug effects; physiology; Rats; Rats, Sprague-Dawley; Serum; Stress, Mechanical
- From: China Journal of Orthopaedics and Traumatology 2013;26(2):142-146
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study influence of serum containing Liuwei Dihuang decoction (see text) on proliferation and differentiation of osteoblast form neonatal SD rats cultured in vitro at different times and different stretch stress.
METHODSAfter osteoblast cultured for 24 hours in the serum containing Liuwei Dihuang decoction (see text) and serum in control group, the 0.5 Hz frequency, 6% and 12% stretch-stress were added. The MTT1 and the activity of ALP were measured at the 12th and 24th hours, and the data were analyzed.
RESULTS1. In the environment of stretch stress to the frequency of 0.5 Hz, and stretched for 24 hours, the osteoblast was stimulated under elongation rate of 6% and 12%; the proliferation and differentiation of osteoblast was more active under elongation rate of 12% than that of 6%. 2. There were no stimulating effects on osteoblast proliferation and differentiation of serum containing Liuwei Dihuiang decoction (see text) acted on osteoblast cells of SD rats cultured in vitro for a shot time.
CONCLUSIONStretch stress environment can enhance osteoblast proliferation and differentiation cultured in vitro.
