Effects of geniposide on SNP-induced apoptosis of chondrocyte and cell cycle.
- Author:
Wan-Jun CHEN
1
;
Tong-Zhu BAO
;
Ken CHEN
;
Chang-Mou ZHU
;
Feng WAN
;
Yu-Lin TAN
;
Fei YAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Cycle; drug effects; Chondrocytes; drug effects; physiology; Female; Iridoids; pharmacology; therapeutic use; Male; Nitroprusside; pharmacology; Osteoarthritis; drug therapy; Rats; Rats, Sprague-Dawley
- From: China Journal of Orthopaedics and Traumatology 2013;26(3):232-235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.
METHODSThe chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by assay; flow cytometer were adopted to observe cell cycle and apoptosis rate; NO examination adopted nitrate reductase method.
RESULTSGeniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly (r=0.917, P<0.01).
CONCLUSIONGeniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mechanism of Geniposide preventing osteoarthritis.
