Effects of recombinant human osteoprotegerin and recombinant RANK protein on the differentiation of osteoclast precursors.
- Author:
Qi XIONG
1
;
Li-Cheng ZHANG
;
Li-Hai ZHANG
;
Qi YAO
;
Peifu TANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Cells, Cultured; Mice; Osteoclasts; cytology; drug effects; Osteoprotegerin; pharmacology; Receptor Activator of Nuclear Factor-kappa B; pharmacology; Recombinant Proteins; pharmacology; Stem Cells; cytology; drug effects
- From: China Journal of Orthopaedics and Traumatology 2013;26(4):324-327
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the effect of recombinant OPG-Fc and recombinant RANK protein on the differentiation of osteoclast precursors.
METHODSMouse osteoblasts cell lines were incubated with osteoclast precursors cell lines RAW 264.7 for 9 days with 10(-5) g/L rhRANK or rhOPG-Fc or PBS added to the coculture system. TRAP stain positive cells counting and cortical bone pit formation counting were performed in the 9th day.
RESULTSMultinuleated TRAP stain positive cells were observed in the cocluture systems after 6 days incubation,and plenty of mature osteoclasts could be observed in the 9th day. With the addition of 10(-5) g/L rhOPG-Fc or rhRANK, multinucleated giant cells and cortical bone pit formation couting decreased significantly compared with the control group, and the rhRANK group decreased more significantly than the rhOPG-Fc group.
CONCLUSIONSBoth rhOPG-Fc and rhRANK can inhabit the differentiation of osteoclast precursors and prevent them forming mature osteoclasts,moreover,the rhRANK shows the significant inhabition effect than the rhOPG-Fc.
