Anti-thrombosis effect and its mechanism of Qingkailing injection.
- Author:
Chunyu CAO
1
;
Aihua LIANG
;
Yong ZHAO
;
Ting LIU
;
Chunying LI
;
Yan YI
;
Jinhua WANG
;
Baoyun XUE
;
Timothy M CHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD11 Antigens; genetics; metabolism; CD18 Antigens; genetics; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Fibrinolytic Agents; administration & dosage; Gene Expression; drug effects; Humans; Injections, Intraperitoneal; Interleukin-6; blood; Male; Random Allocation; Rats; Rats, Sprague-Dawley; Thrombosis; drug therapy; genetics; metabolism; Tumor Necrosis Factor-alpha; blood
- From: China Journal of Chinese Materia Medica 2009;34(12):1549-1552
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-thrombosis effect and its mechanism of Qingkailing injection (QKL).
METHODSD rats were randomly divided into control group, model group and QKL 2.5, 5.0, 10 groups. QKL were given (i.p.) to rats once a day for successively 4 days. The rats in all groups but control were pretreated with carrageenin (Ca) i.p. at 16 h before the last dose of QKL and followed by intravenous injection of endotoxin ( LPS fom E. coli O111:B4) 50 microg x kg(-1) 30 min after the last dosing of QKL. Thrombosis in rat tails were observed at 24 h after injection of LPS. The number of white blood cells and platelets, serum TNF-alpha, IL-6 level, CD11b/CD18 expression of white blood cells and platelet aggregation were analysed.
RESULTQKL obviously inhibited the LPS/Ca-induced thrombosis as showed a reduced infarction range due to thrombosis in tails. The sera concentration of TNF-alpha and IL-6, expression of CD11b/CD18 in WBC and platelet coagulation rate were reduced after QKL treatment.
CONCLUSIONThe anti-thrombosis action of QKL is associated with inhibition of WBC activation and adherence, reduction of inflammatory factor release and abating of platelet coagulation rate. The anti-thrombosis mechanism of QKL is consistent with its function of clearing away heat-evil and toxic materials.
