Identification of differentially expressed genes in different anomalies of Glycyrrhiza uralensis by cDNA-AFLP method.
- Author:
Quan YANG
1
;
Shengli WEI
;
Wenquan WANG
;
Chunsheng LIU
;
Chunrong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; DNA, Complementary; genetics; Gene Expression Profiling; Gene Expression Regulation, Plant; Glycyrrhiza uralensis; genetics; Molecular Sequence Data; Plant Proteins; genetics; Plant Roots; genetics
- From: China Journal of Chinese Materia Medica 2009;34(13):1628-1631
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand the molecular mechanism in genetic variation within species, and identify the differentially expressed genes among 8 anomalies of Glycyrrhiza uralensis under the same genetic background and cultivated environment.
METHODThe differentially expressed genes in roots of different anomalies of G. uralensis in vigorous growing stage were identified by cDNA-AFLP and confirmed by reverse-Northern hybridization. Their functions were inferred through bioinformatics method.
RESULTSFourteen differentially expressed genes were identified, among which, the function of 6 were unknown, and the rest were involved in antibacterium, regulating metabolism, enhancing the heat resistance ability, increasing the nitrogen fixation ability, and other bioprocesses.
CONCLUSIONFor the first time, the differentially expressed genes were cloned from the different anomalies of G. uralensis. This provided the basis for the screening of fine anomalies and varieties, and the research of functional genomics.
