Accumulation of flavonoids and chlorogenic acid in callus and suspension cell of Eucommia ulmoides.
- Author:
Zhibin ZHANG
1
;
Riming YAN
;
Xiaofang QIU
;
Qinggui ZENG
;
Hai YOU
;
Du ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; Cells, Cultured; Chlorogenic Acid; analysis; metabolism; Eucommiaceae; chemistry; metabolism; Flavonoids; analysis; metabolism
- From: China Journal of Chinese Materia Medica 2009;34(13):1636-1639
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze and compare the cell growth and accumulation of flavonoids and chlorogenic acid in the callus and suspension cell of Eucommia ulmoides.
METHODThe callus induced from the leaf of E. ulmoides seedlings were suspended in liquid medium. The time courses of cell growth and yields of flavonoids and chlorogenic acid were studied.
RESULTThe highest contents of flavonoids and chlorogenic acid in the callus were 13.46, 1.712 mg x g(-1), respectively, while the contents of these two secondary metabolites were 16.63, 3.93 mg x g(-1) in suspension cell culture correspondingly.
CONCLUSIONComparing with callus, the suspension cell showed a short growth period and high growth rate with a remarkable high content of flavonoids and chlorogenic acid.
