Isolation and determination of anamorph of Shiraia bambusicola.
- Author:
Du WEN
1
;
Yanfeng HAN
;
Jiandong LIANG
;
Zongqi LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Ascomycota; classification; genetics; isolation & purification; DNA, Fungal; genetics; DNA, Ribosomal Spacer; genetics; Molecular Sequence Data; Mycological Typing Techniques; Phylogeny; RNA, Ribosomal, 5.8S; genetics
- From: China Journal of Chinese Materia Medica 2009;34(13):1640-1642
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and identify the anamorph of Shiraia bambusicola.
METHODFungus strains were isolated from mature ascospores and stroma. They were identified by means of morphological identification including colony and microscope characteristic, the molecular identification was done by 5.8S-ITS rDNA.
RESULTThe strains GZDXIFR-171 and GZDXIFR-181 were isolated and obtained with the separation of different methods, which had the same colony morphology. With the universal primers of the ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequence of GZDXIFR-171 and GZDXIFR-181 were obtained by the PCR amplification and sequencing. Compared with the published nucleotide sequence of 5.8S-ITS rDNA in NCBI (National Center for Biotechnology Information), GZDXIFR-171 and GZDXIFR-181 were highly identical with S. bambusicola.
CONCLUSIONThe isolated strains GZDXIFR-171 and GZDXIFR-181 were confirmed to be the true anamorph of S. bambusicola.
