Pre-treatment in determining total polysaccharide in flos Chrysanthemum indicum by response surface design.

Author: Hailing FANG ¹ ; Qiaosheng GUO ; Haijin SHEN
Author Information

1. Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.
Publication Type:Journal Article
MeSH: Analytic Sample Preparation Methods; Chrysanthemum; chemistry; Flowers; chemistry; Plant Extracts; chemistry; Polysaccharides; analysis
From: China Journal of Chinese Materia Medica 2009;34(13):1665-1667
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici.
METHODThe factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design.
RESULTThe best extraction condition as follows: 81.0 degrees C of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best.
CONCLUSIONA model equation that can be used to predict the experiment is established through the response surface method.