Identification and comparison of constituents in HPLC fingerprint of five Chinese herbal pieces from Rheum palamatum.
- Author:
Li LI
1
;
Cun ZHANG
;
Yongqing XIAO
;
Na LIN
;
Chunfang LIU
;
Zhen PANG
;
Guiliu LI
;
Dongdong CHEN
;
Guofang TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; Rheum; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(13):1668-1671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identificate and compare constituents in the HPLC fingerprint of five Chinese herbal pieces from Radix et Rhizoma Rhei.
METHODHPLC analysis was carried out with methyl alcohol 1% glacial acetic acid as gradient elution, changes in five Chinese herbal pieces and medicinal material under 280 nm and 430 nm were compared.
RESULTHPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces were similar, 24 peaks were identified under 280 nm 19 or 22 peaks could be indicated in the braising with liquor and the charring respectively. Under 430 nm, 8 peaks were identified except the braising with liquor.
CONCLUSIONHPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces are similar while the changes on chemical composition and the content in braising with liquor and the charring are remarkable.
