Identification on Syngnathus and its adulterants with random amplified polymorphic DNA.
- Author:
Yan WU
1
;
Jia LIU
;
Mengyue WANG
;
Peijun JU
;
Xiaobo LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; DNA Primers; genetics; Phylogeny; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique; Smegmamorpha; classification; genetics
- From: China Journal of Chinese Materia Medica 2009;34(14):1758-1760
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the identification method of Syngnathus and its adulterants.
METHODRandom amplified polymorphic DNA (RAPD) was used to construct a dendrogram by UPGMA method based on Nei & Li's coefficient and a genetic affinity pattern for Syngnathus acus, Solenognathus hardwickii, Syngnathoides biaculeatus, Trachyrhamphus serratus, Halicampus koilomatodon, Microphis boaja.
RESULTFour primers, LJ04, LJ09, LJ16 and LJ19, from 18 random primers were used in the dendrogram which can differentiate Syngnathus in genus level and showed a great consistence with the appearance identification. The genetic affinity pattern based on primers LJ09 and LJ19 could be used to identify Syngnathus from its adulterants.
CONCLUSIONRAPD is suitable to identify Syngnathus and its adulterants.
