Agrobacterium tumefaciens mediated Chitinase and beta-1,3-glucanase gene transformation for Pinellia ternata.
- Author:
Bo JIN
1
;
Fusheng JIANG
;
Meirong YU
;
Nipi CHEN
;
Zhishan DING
Author Information
- Publication Type:Journal Article
- MeSH: Agrobacterium tumefaciens; genetics; metabolism; Chitinases; genetics; metabolism; Fungal Proteins; genetics; metabolism; Gene Expression Regulation, Plant; Gene Transfer Techniques; Genetic Vectors; genetics; metabolism; Glucan 1,3-beta-Glucosidase; genetics; metabolism; Pinellia; genetics; metabolism; Transformation, Genetic; Trichoderma; enzymology
- From: China Journal of Chinese Materia Medica 2009;34(14):1765-1767
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.
METHODUsing hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.
RESULTPCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.
CONCLUSIONThe results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata
