Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration.

Author: Shihua SONG ¹ ; Xiaobin JIA ; Yan CHEN ; Lijing WANG ; Liang FENG
Author Information

1. Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Publication Type:Journal Article
MeSH: Chromatography, Supercritical Fluid; methods; Ergosterol; analysis; isolation & purification; Fruit; chemistry; Plant Extracts; analysis; isolation & purification; Reishi; chemistry
From: China Journal of Chinese Materia Medica 2009;34(14):1783-1785
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the feasibility of supercritical fluid extraction (SFE) for extraction of ergosterols from the fruits of Ganoderma lucidum.
METHODOptimum extraction conditions were studied by orthogonal tests, the ergosterol were analyzed by RP-HPLC.
RESULTThe optimal extraction conditions were pressure as 20 MPa, temperature at 45 degrees C, using ethanol as modifier carrier at the volume of 1 mL x g(-1), and 1.5 hour of extraction time.
CONCLUSIONSFE has the superiority of adjustable polarity, and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.