Chemical constituents and antitumor activity on leukemia K562 cell of Leonurus heterophyllus.
- Author:
Yue CONG
1
;
Jinggong GUO
;
Tianxiao WANG
;
Mian LI
;
Ke LI
;
Jinhui WANG
;
Qin LI
Author Information
1. Institute of Traditional Chinese Materia Medica, School of Pharmacy, Henan University, Kaifeng 475004, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
chemistry;
isolation & purification;
pharmacology;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
pharmacology;
Humans;
K562 Cells;
Leonurus;
chemistry
- From:
China Journal of Chinese Materia Medica
2009;34(14):1816-1818
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of Leonurus heterophyllus were separated and purified by repeated column chromatography on silica gel, HPD 100, Sephadex LH-20, and PHPLC. Each compound was characterized by spectroscopic and physical data. Eight compounds have been purified and identified to be quercetin 3-O-robinobioside (1), rutin (2), isoquerci trin (3), hyperoside (4), quercetin (5), apigenin (6), genkwanin (7), and benzoic acid (8). Among them, compounds 2, 5-7 were isolated from L. heterophyllus for the first time; Compounds 1, 3, 4, 8 were obtained for the first time from the genus Leonurus. The in vitro activities against leukemia K562 Cells of pure components were evaluated by testing their IC50. Compounds 1-6, 8 exhibited in-vitro inhibitory activities against leukemia K562 cells in different extent.
