Comparison of content of clemastanin B of Radix Isatidis in different growing areas.

Author: Yiqiang AN ¹ ; Xiaobin JIA ; Lili CHANG ; Feng SHI
Author Information

1. Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Key Laboratory of Scientfic Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. anyhe2006@yahoo.com.cn
Publication Type:Journal Article
MeSH: China; Furans; analysis; Isatis; chemistry; Plant Extracts; analysis
From: China Journal of Chinese Materia Medica 2009;34(14):1823-1825
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo develop an HPLC method for determination of clemastanin B which has anti-viral activity in Radix Isatidis and compare the contents of clemastanin B in the drugs from different origins.
METHODThe samples were separated on an ZORBAX SB-C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (11:89). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 225 nm. Column temperature was 30 degrees C.
RESULTThe linear range of clemastanin B was 0.0615-1.8441 microg (r = 0.9995), the average recovery was 97.74%, RSD was 1.4% (n=9). The contents of clemastanin B were in the range of 0.269-0.900 mg x g(-1) in Radix Isatidis from different origins.
CONCLUSIONThe method for quantitation of clemastanin B in Radix Isatidis was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis.