Hairy root culture optimization and aucubin medical composition production of Eucommia ulmoides.
- Author:
Rui YANG
1
;
Jiahui ZHANG
;
Xuemei SUN
;
Min SUN
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; metabolism; Eucommiaceae; chemistry; growth & development; metabolism; Glucosides; analysis; metabolism; Iridoid Glucosides; Iridoids; analysis; metabolism; Plant Roots; chemistry; growth & development; metabolism; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2009;34(15):1902-1905
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots.
METHODHairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC.
RESULTThe best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots.
CONCLUSIONHairy root of E. ulmoides will be useful for the production of active components in aucubin.
