Synthesis and optimize reaction conditions of chlorogenic acid artificial antigen.
- Author:
Hui LIN
1
;
Ping LIU
;
Jianxun WANG
;
Bin LI
;
Lihua MU
;
Yuan HU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Plant; chemistry; Chlorogenic Acid; chemical synthesis; chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet
- From: China Journal of Chinese Materia Medica 2009;34(15):1906-1909
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo synthesize artificial antigen of the chlorogenic acid (CGA), a constituent in Traditional Chinese Medicine, then optimize the reaction conditions, so that to supply the basis in material and technique for study on Chinese medicine injection of adverse reactions in-depth.
METHODThe complete antigen of chlorogenic acid (CGA-BSA) was synthesized by CGA coupled to carrier protein bovine serum albumi (BSA) using carbodiimide method. We used the orthogonal design test to optimize reaction conditions and used the MALDI-TOF-MS and UV motheds to determine the coupling rate of the CGA-BSA.
RESULTThe coupling rate were 20 and 18.8, counting by MALDI-TOF-MS and UV, respectively; while the best reaction conditions were NHS: CGA, EDC: CGA, CGA: BSA were 4: 1, 1: 1, 200: 1, respectively, with the pH 9.
CONCLUSIONThe complete antigen is preparated successfully. Which supplied the basis to advanced study of correlation.
