Generation of high affinity humanized single-chain antibody against PreS1 of hepatitis B virus from immune phage displayed antibody library.

Author: Zhi Chao ZHANG ¹ ; Qi LI ; Xue Jun HU
Author Information

1. Department of Bioengineering, Dalian University of Technology, Dalian 116012, China.
Publication Type:Journal Article
MeSH: Antibodies, Monoclonal; biosynthesis; Antibody Affinity; Hepatitis B Antibodies; biosynthesis; Hepatitis B Surface Antigens; immunology; Humans; Peptide Library; Protein Precursors; immunology
From: Chinese Journal of Hepatology 2003;11(2):90-92
CountryChina
Language:Chinese
Abstract:
OBJECTIVEScreening and characterizing high affinity completely humanized single-chain antibody (ScFv) against PreS1 of hepatitis B virus.
METHODSA combinatorial library of phage-displayed human ScFv, genes of which were derived from peripheral blood lymphocytes immunized by PreS1 of Hepatitis B Virus in vitro, was constructed. The library contained 7 10(8) clones.
RESULTSAfter 3 rounds panning, a high affinity (K=10(7) to 10(8) mol/L) ScFv specific to PreS1 was obtained. The V(H) belonged to human V(H4) family, and V(1) to V(4) by sequence analysis.
CONCLUSIONThis study suggests that the method of antigen stimulation in vitro is an expeditious way for the source of human immune antibody. And the ScFv may provide a more satisfactory therapy.