Preparation and application of the monoclonal antibody against hepatoma-specific gamma-glutamyltransferase.
- Author:
Wei TANG
1
;
Xiao Ying WANG
;
Jian Ee GAO
;
He Ping JI
;
Qi Min TAO
;
Ying JI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; Carcinoma, Hepatocellular; diagnosis; enzymology; Enzyme-Linked Immunosorbent Assay; Female; Liver Neoplasms; diagnosis; enzymology; Mice; Mice, Inbred BALB C; gamma-Glutamyltransferase; analysis; immunology
- From: Chinese Journal of Hepatology 2003;11(2):100-102
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare a monoclonal antibody against hepatoma-specific gamma-glutamyltransferase (GGT-II) and study it's application.
METHODSTwo Bal B/C mice were immunized with pure GGT-II, then their spleen cells were separated and fused to SP 2/0 myeloma cells so as to make hybridoma cell strain which could yield monoclonal antibody against GGT-II. And it's effect of binding GGT-II was detected by competitive inhibitory enzyme linked-immunosorbance assay (ELISA).
RESULTSA mouse hybridoma cell strain which could steadily secrete the monoclonal antibody against GGT-II was obtained and named 2G4F6B2. This monoclonal antibody belonged to IgG1 subclass and was specific to GGT-II, without cross-reaction to GGT-II. The result of detecting human serum GGT-II by ELISA with the monoclonal antibody accorded with that by polyacrylamide gradient electrophoresis.
CONCLUSIONThe monoclonal antibody against GGT-II prepared in this study has high specificity and can be applied in clinic to detect human serum GGT-II.
