Expression of MAGE, GAGE, and BAGE genes in human hepatocellular carcinoma.
- Author:
Jun-bin SHAO
1
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Neoplasm; biosynthesis; genetics; Carcinoma, Hepatocellular; genetics; pathology; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms; genetics; pathology; Melanoma-Specific Antigens; Neoplasm Proteins; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2003;11(3):142-144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of MAGE, GAGE and BAGE genes in human hepatocellular carcinoma (HCC) cell lines.
METHODSThe expression levels of MAGE-1, MAGE-3, GAGE1-8, GAGE1-2 and BAGE mRNAs in HCC cell lines SMMC-7721, QQY-7701, BEL-7402 were studied by reverse transcription polymerase chain reaction and were compared with those in biopsied liver tissues.
RESULTSMAGE-1 and BAGE mRNAs were expressed in SMMC-7721 cells. MAGE-3 and BAGE mRNAs were expressed in QQY-7701 cells. MAGE-1 and GAGE1-2 mRNAs were expressed in BEL-7402 cells. None of these genes was expressed in biopsied liver tissues.
CONCLUSIONSMAGE-1, MAGE-3, GAGE1-8, GAGE1-2 and BAGE are expressed in hepatocellular carcinoma cell lines. These tumor-specific antigens can be used as molecular markers for early diagnosis and possible targets for immunotherapy of human HCC.
