Preliminary study on hepatitis B virus quasispecies in a patient with chronic hepatitis B.
- Author:
Lin LAN
1
;
Yu-ming WANG
;
Yan-ping HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Hepatitis B Core Antigens; genetics; immunology; Hepatitis B virus; classification; genetics; isolation & purification; Hepatitis B, Chronic; virology; Humans; Male; Mutation; Promoter Regions, Genetic; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Hepatology 2003;11(4):219-221
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether the hepatitis B virus (HBV) has quasispecies character by studying nucleotide sequence polymorphism and mutation features of HBV PreC/C gene region, and preliminaryly explore the heterogeneity of HBV quasispecies.
METHODSThe serum sample was obtained from a patient with chronic hepatitis B, and the whole HBV PreC/C gene region was amplified by PCR and cloned. Thirty-four clones that contained HBV PreC/C gene fragments were sequenced.
RESULTSThere were 28 kinds of different nucleotide sequences in 34 clones, and the nucleotide sequences diversity ranged from 0.2% to 2.1%. The mutation points were almost distributed in the whole region, but there wasn't mutation at PreC region nt.1 896 point in all sequences.
CONCLUSIONHepatitis B virus has complex quasispecies character in the patients with chronic hepatitis B.