Hepatitis B virus genotypes and the heterogeneity of its polymerase gene.
- Author:
Guo-bing ZENG
1
;
Shu-juan WEN
;
Jian SUN
;
Ze-yan GUO
;
Zhan-hui WANG
;
Kang-xian LUO
;
Li YAN
;
Jin-lin HOU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; DNA, Viral; genetics; DNA-Directed DNA Polymerase; genetics; Gene Products, pol; genetics; Genetic Heterogeneity; Genotype; Hepatitis B virus; genetics; physiology; Humans; Molecular Sequence Data; Mutation; Phylogeny
- From: Chinese Journal of Hepatology 2003;11(5):265-267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the heterogeneity of polymerase gene (P gene) within hepatitis B virus (HBV) genotypes based on a systematic analysis of 202 HBV P genes, providing some useful references for further studies on the relationship among HBV genotypes, P gene mutations, replication and nucleoside analogues drug-resistance.
METHODS202 HBV complete sequences containing P genes were obtained from GenBank and were analysed using computer softwares.
RESULTSThere were some genotype-related characteristics of HBV P genes. As reverse transcriptase domain was concerned, there were more amino acid divergences in genotype C and D compared with these in genotype A. There were also amino acid substitutions in the A-F conserved regions of the reverse transcriptase domain within and between HBV genotypes.
CONCLUSIONSThere are divergences of P genes and amino acids within and between HBV genotypes, which should be considered when amino acid changes are analyzed whether they are proposed to be drug-resistance mutations or the results from quasispecies-selected. Moreover, these divergences may affect the antiviral effect of nucleoside analogues on HBV with different genotypes.
