Heat shock protein 70-HBsAg complex inducing antigen-specific cytotoxic T lymphocyte immune response.
- Author:
Ming-li PENG
1
;
Hong REN
;
Hong-mei XU
;
Min CHEN
;
Ning LING
;
Yu-ling QIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Dendritic Cells; immunology; secretion; Female; HSP70 Heat-Shock Proteins; genetics; immunology; Hepatitis B Surface Antigens; genetics; immunology; Humans; Immunization; Mice; Mice, Inbred BALB C; Mycobacterium tuberculosis; chemistry; Peptides; chemistry; T-Lymphocytes, Cytotoxic; immunology
- From: Chinese Journal of Hepatology 2003;11(5):271-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possibility of cell-medicated immune response induced with heat shock protein 70 (HSP70)-HBsAg protein complex in vitro.
METHODSHSP70-HBsAg complex was reconstituted in vitro which was injected into mice in order to observe that whether HSP70-HBsAg would stimulate humoral and cellular immune responses. HSP70, HSP70-HBsAg complex and HBsAg were used to activate the dentritic cell (DC) individually, which would initiate homogeneic T lymphocyte to transform to cytotoxic T lymphocyte (CTL). The cytotoxicity of CTL was detected with MTT assay.
RESULTSHSP70-HBsAg complex elicited both humoral and cellular immune responses against HBsAg in mice. Specific CD8+ CTL response was readily induced by HSP70-HBsAg complex and HBsAg, especially the former.
CONCLUSIONSHSP70-HBsAg complex is immunogenic and HSP70 can lead to great efficient CTL response. And HSP70-HBsAg complex may be used as a protein vaccine for immunotherapy for chronic hepatitis B.
