Effect of blocking transforming growth factor beta signalling on culture-activated rat hepatic stellate cells.
- Author:
Ya-jun ZHOU
1
;
Dong-mei YIN
;
Hong-shan CHEN
;
Hui-xia ZHU
;
Xin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Cell Division; drug effects; Cells, Cultured; Collagen Type I; biosynthesis; genetics; Gene Transfer Techniques; Genetic Vectors; Liver; drug effects; pathology; physiology; Liver Cirrhosis; pathology; Rats; Rats, Sprague-Dawley; Signal Transduction; Transforming Growth Factor beta; pharmacology
- From: Chinese Journal of Hepatology 2003;11(5):282-284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of blocking transforming growth factor beta (TGF-beta) signalling on culture-activated rat hepatic stellate cells (HSCs).
METHODSAfter cultured in plastic dish for two days, HSCs were infected with adenovirus vector AdT beta-ExR or AdLacZ (control) at 10 multiplicity of infection (MOI) and incubated for four days. The expression of type I collagen, alpha-smooth muscle actin (alpha-SMA) and the proliferation of HSCs were analyzed by ELISA, western blot, immunocytochemistry and BrdU uptake respectively.
RESULTSThe expression level of type I collagen in HSCs infected with AdT beta-ExR was 42.99% of that in HSCs infected with AdLacZ (q = 9.100, P < 0.001). The expression of alpha-SMA in HSCs infected with AdTbeta-ExR was also inhibited evidently. But the BrdU uptake in HSCs infected with AdLacZ was 49.24% of that in HSCs infected with AdTbeta-ExR (q = 7.835, P < 0.001).
CONCLUSIONSThe blockade of TGF-beta signalling in cultured rat HSCs can inhibit their activation significantly, but promote their proliferation.
